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p 2000 laser pipette puller  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company p 2000 laser pipette puller
    P 2000 Laser Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+pipette/P-2000%2FG+Laser-Based+Glass+Puller/pm42815478-111-11-15
    Average 93 stars, based on 57 article reviews
    p 2000 laser pipette puller - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Transferring:

    Article Title: Extant life detection using label-free video microscopy in analog aquatic environments.
    Article Snippet: .. This glass pipette was pulled on a Sutter Instruments P-97 puller using “patch clamp” settings. ..

    Article Title: Long-term training alters response dynamics in the aging auditory cortex.
    Article Snippet: Age-related auditory dysfunction, presbycusis, is caused in part by functional changes in the auditory cortex (ACtx) such as altered response dynamics and increased population correlations.. Given the ability of cortical function to be altered by training, we tested if performing auditory tasks might benefit auditory function in old age.. We examined this by training adult mice on a low-effort tone-detection task for at least six months and then investigated functional responses in ACtx at an older age (~18 months).

    Article Title: Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide
    Article Snippet: .. For monosynaptic retrograde tracing, 60–80 nL of a mixture (1:1) of AAV2/9-rAAV-EF1α-DIO-oRVG-WPRE-hGH pA (5.40 × 10 12 genomic copies per mL, BrainVTA, Wuhan, China) and AAV2/9-rAAV-EF1α-DIO-H2B-EGFP-T2A-TVA-WPRE-hGH pA (5.24 × 10 12 genomic copies per mL, BrainVTA) is unilaterally injected into the LPB at 40 nL/min using a single syringe infuse/withdraw system (KD Scientific, Holliston, MA, USA) connected to a glass pipette (tip diameter 10–30 μm, Sutter Instrument, Novato, CA, USA) (Fig. C–E). ..

    Article Title: Creating Alternative Afferent Input to Facilitate the Regeneration of Injured Primary Afferent Neurons
    Article Snippet: .. Injection of synapse organizer into the spinal trigeminal nucleus caudalis One microliter of physiological saline or 1 μL of synapse organizer (1 μg/μL) was administered to the caudal part of the spinal trigeminal nucleus using an electronic microinjector (BJ-110 BEX; Bex Corporation, Toyota City, Japan) and a glass pipette made with a micropipette puller (P-97IVF; Sutter Instrument Co., Novato, CA, USA). ..

    Article Title: Using a micro-device with a deformable ceiling to probe stiffness heterogeneities within 3D cell aggregates
    Article Snippet: .. The cantilever consists of an 80 μm diameter flexible nitinol fiber (NitiWire, Fort Waynes Metals) attached to a solid L-shaped glass pipette (Sutter instrument), held in place using a pipette holder (Narishige). ..

    Article Title: Supporting Information for Anion efflux mediates transduction in the hair cells of the zebrafish lateral line
    Article Snippet: The permeability of the cupular matrix was measured by recording the penetration of a negatively-charged fluorophore (6-carboxyfluorescein; Sigma-Aldrich) into the cupula. .. The fluorophore (10 μM in HEPES-buffered E3) was loaded into a glass pipette (tip diameter: ~5 μm; Model P-97 Flaming/Brown Micropipette Puller, Sutter Instrument Co.) aimed ~20 μm away at the cupula. .. The pipette pressure was then rapidly increased by opening a valve attached to a pressure source (100 torr, Fluke Biomedical Instruments DPM1B), dye surrounded the cupula, and the change in fluorescence over time was recorded at the stereocilia level (Leica TCS SP5, HCX PL APO CS 63x/1.2 water objective, 20 frames/sec, excitation: 488 nm; emission: 500 - 550 nm).


    Patch Clamp:

    Article Title: Extant life detection using label-free video microscopy in analog aquatic environments.
    Article Snippet: .. This glass pipette was pulled on a Sutter Instruments P-97 puller using “patch clamp” settings. ..

    Injection:

    Article Title: Long-term training alters response dynamics in the aging auditory cortex.
    Article Snippet: Age-related auditory dysfunction, presbycusis, is caused in part by functional changes in the auditory cortex (ACtx) such as altered response dynamics and increased population correlations.. Given the ability of cortical function to be altered by training, we tested if performing auditory tasks might benefit auditory function in old age.. We examined this by training adult mice on a low-effort tone-detection task for at least six months and then investigated functional responses in ACtx at an older age (~18 months).

    Article Title: Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide
    Article Snippet: .. For monosynaptic retrograde tracing, 60–80 nL of a mixture (1:1) of AAV2/9-rAAV-EF1α-DIO-oRVG-WPRE-hGH pA (5.40 × 10 12 genomic copies per mL, BrainVTA, Wuhan, China) and AAV2/9-rAAV-EF1α-DIO-H2B-EGFP-T2A-TVA-WPRE-hGH pA (5.24 × 10 12 genomic copies per mL, BrainVTA) is unilaterally injected into the LPB at 40 nL/min using a single syringe infuse/withdraw system (KD Scientific, Holliston, MA, USA) connected to a glass pipette (tip diameter 10–30 μm, Sutter Instrument, Novato, CA, USA) (Fig. C–E). ..

    Article Title: Creating Alternative Afferent Input to Facilitate the Regeneration of Injured Primary Afferent Neurons
    Article Snippet: .. Injection of synapse organizer into the spinal trigeminal nucleus caudalis One microliter of physiological saline or 1 μL of synapse organizer (1 μg/μL) was administered to the caudal part of the spinal trigeminal nucleus using an electronic microinjector (BJ-110 BEX; Bex Corporation, Toyota City, Japan) and a glass pipette made with a micropipette puller (P-97IVF; Sutter Instrument Co., Novato, CA, USA). ..


    Retrograde Tracing:

    Article Title: Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide
    Article Snippet: .. For monosynaptic retrograde tracing, 60–80 nL of a mixture (1:1) of AAV2/9-rAAV-EF1α-DIO-oRVG-WPRE-hGH pA (5.40 × 10 12 genomic copies per mL, BrainVTA, Wuhan, China) and AAV2/9-rAAV-EF1α-DIO-H2B-EGFP-T2A-TVA-WPRE-hGH pA (5.24 × 10 12 genomic copies per mL, BrainVTA) is unilaterally injected into the LPB at 40 nL/min using a single syringe infuse/withdraw system (KD Scientific, Holliston, MA, USA) connected to a glass pipette (tip diameter 10–30 μm, Sutter Instrument, Novato, CA, USA) (Fig. C–E). ..

    Saline:

    Article Title: Creating Alternative Afferent Input to Facilitate the Regeneration of Injured Primary Afferent Neurons
    Article Snippet: .. Injection of synapse organizer into the spinal trigeminal nucleus caudalis One microliter of physiological saline or 1 μL of synapse organizer (1 μg/μL) was administered to the caudal part of the spinal trigeminal nucleus using an electronic microinjector (BJ-110 BEX; Bex Corporation, Toyota City, Japan) and a glass pipette made with a micropipette puller (P-97IVF; Sutter Instrument Co., Novato, CA, USA). ..

    other:

    Article Title: Opto-juxtacellular interrogation of neural circuits in freely moving mice.
    Article Snippet: Neural circuits are assembled from an enormous variety of neuronal cell types.. Although significant advances have been made in classifying neurons on the basis of morphological, molecular and electrophysiological properties, understanding how this diversity contributes to brain function during behavior has remained a major experimental challenge.. Here, we present an extension to our previous protocol, in which we describe the technical procedures for performing juxtacellular opto-tagging of single neurons in freely moving mice by using Channelrhodopsin-2–expressing viral vectors.



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